Serial Blockface SEM, FIB-SEM and some serial sectioning TEM approaches use common workflows. Here we describe the general features, with links to some specific approaches and methods you may like to start off with.
Fixation – how to perfuse animals or provide other fixation
Staining – heavy metal staining reasons and methods
Mounting – for VolumeScope, for 3View, for FIB-SEM. (Orange = coming)
Imaging – some starting settings and considerations and calcs
PostProcessing and Storage
Tracing and measuring data – software etc