A Simplified Large Volume 3DEM Workflow

Overview

Serial Blockface SEM, FIB-SEM and some serial sectioning TEM approaches use common workflows.  Here we describe the general features, with links to some specific apporaches and methods you may like to start off with.

Fixation – how to perfuse animals or provide other fixation

Staining – heavy metal staining reasons and methods

Mounting – for VolumeScope, for 3View, for FIB-SEM.  (Orange = coming)

Imaging – some starting settings and considerations and calcs

PostProcessing and Storage

Tracing and measuring data – software etc