Claudia Lopez

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  • in reply to: Biowave users? #574
    Claudia LopezClaudia Lopez
    Participant

    Just FYI, we have tried most of our runs in pancreas, not brain.

    in reply to: Biowave users? #573
    Claudia LopezClaudia Lopez
    Participant

    Hi Emily,
    We have used the Biowave for SBF-SEM but found that the staining is not even along the tissue. We use Biowave for all of our TEM samples with great success and for some samples run on FIB-SEM but we are still optimizing the protocol for SBF. We definitely need to modify the times used on the Biowave for each step and check if this will result in an even staining. We have tried different tissue sizes and even in the smallest one we can see like a banding pattern of staining. Once we optimize the protocol we can share it, so far it has not worked for us.
    Claudia

    in reply to: do we want a chat facility too? #526
    Claudia LopezClaudia Lopez
    Participant

    Hi Grahame, thanks for organizing this website!

Viewing 3 posts - 1 through 3 (of 3 total)