We have used the Biowave for SBF-SEM but found that the staining is not even along the tissue. We use Biowave for all of our TEM samples with great success and for some samples run on FIB-SEM but we are still optimizing the protocol for SBF. We definitely need to modify the times used on the Biowave for each step and check if this will result in an even staining. We have tried different tissue sizes and even in the smallest one we can see like a banding pattern of staining. Once we optimize the protocol we can share it, so far it has not worked for us.
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