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email from the users list in ultimate member plugin needs help
Of course the FIB sets are much smaller so less of an issue.
Our standard VolumeScope imaging run would be about 24 hrs, in which we’d collect between about 30GB and 150 GB of raw data. Good staining=faster scans, more data.
We keep raw data intact and make a second set of 8Bit Tiff images, plus scaled-down (50% and 25%) versions. And we also make enhanced versions of the data – CLAHE filtering and ROF denoising for some work. This about doubles the data – allowing that some of the raw data doesn’t require duplication.
You can compress images. Lossless TIF compression (lzw) works just fine and might save some space. I don’t recommend, but you may also elect to jpeg some of the working datasets. JPEG at high quality can squash down from 2-10 fold without much artifact. But lossy compression (like JPEG) definitely creates structures in the data if you are not careful.
You can compress whole datasets. Zip and tar.gz compression archiving can crunch down your datasets somewhat for storage.
If server space is limited, you may consider archiving raw/all data to tape systems, and maintain only active data on the server. Ask if you want to know more – tapes are cheap and disks die easily.
What do other folk do?
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